文献翻译LEEP手术烟雾中的HPV
01
目的
本研究的目的是探讨LEEP手术产生的烟雾中是否存在人乳头瘤病毒(HPV)DNA。并研究了这些HPVDNA对手术医生的影响。
Thepurposeofthisstudywastoexplorewhetherhumanpapillomavirus(HPV)DNAispresentinsurgicalsmokegeneratedbyloopelectrosurgicalexcisionprocedures(LEEPs).Furthermore,weinvestigatedtheimpactofthisHPVDNAonsurgeons.
02
方法
研究对象包括在年至年期间接受LEEP治疗CIN病变的持续HPV感染的名门诊患者,以及相应的LEEP手术医生。使用流式荧光原位杂交技术(FISH)检测LEEP前后患者脱落的宫颈上皮细胞、手术烟雾及手术医生鼻黏膜上皮细胞中的HPVDNA。
AtotalofoutpatientswithpersistentHPVinfectionstreatedwithLEEPforcervicalintraepithelialneoplasiabetweenand,alongwiththecorrespondingLEEPoperators,wereincluded.TheflowfluorescenceinsituhybridizationtechniquewasusedtodetectHPVDNAinexfoliatedcervicalcellsfromthepatients,insurgicalsmokeandinnasalepithelialcellsfromthesurgeonsbeforeandafterLEEP.
03
结果
上述三种样本中HPVDNA的阳性率分别为94.8%,29.9%和1.5%。手术烟雾中HPV亚型的分布与宫颈标本中的相同。手术烟雾中HPVDNA的阳性率随着抽吸装置距手术部位的距离的增加而显著增加。两名手术医生的鼻黏膜上皮细胞HPVDNA检测呈阳性,且基因型与相应手术烟雾中的基因型一致。经过3-6个月的随访,这两位医生的鼻拭子提示HPVDNA转阴。
ThepositiveratesofHPVDNAinthethreetypesofsamplesmentionedabovewere94.8%,29.9%and1.5%,respectively.ThedistributionofHPVsubtypesinsurgicalsmokewasidenticaltothatinthecervicalspecimens.ThepositiverateofHPVDNAinsurgicalsmokewassignificantlyincreasedforgreaterdistancesofthesuctiondevicefromthesurgicalsite.ThenasalepithelialcellsoftwosurgeonswerepositiveforHPVDNA,andthegenotypeswereconsistentwiththoseinthecorrespondingsurgicalsmoke.Aftera3-6-monthfollow-up,thenasalswabsfromthesetwodoctorstestednegativeforHPVDNA.
04
结论
该研究表明LEEP产生的手术烟雾中存在HPVDNA,并且在手术过程中存在HPVDNA的随空气传播的风险。幸运的是,术者鼻咽部的HPV并不会持续存在。
ThisstudydemonstratedthepresenceofHPVDNAinsurgicalsmokeproducedbyLEEPandtheriskofairbornetransmissionofHPVDNAduringtheoperation.Fortunately,theHPVDNAinthenasopharynxoftheoperatorswasnotpersistent.
参考文献
ZhouQingfeng,HuXiaoli,ZhouJunhan,etal.HumanpapillomavirusDNAinsurgicalsmokeduringcervicalloopelectrosurgicalexcisionproceduresanditsimpactonthesurgeon[J].Cancermanagementandresearch,,11.
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